Monocistronic transcription is the physiological mechanism of sea urchin embryonic histone gene expression.
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چکیده
منابع مشابه
Embryonic regulation of histone ubiquitination in the sea urchin.
We have used quantitative 2-D protein electrophoresis and immunoprecipitation to study the patterns of histone ubiquitination at 10 h and 36 h of embryonic development in Strongylocentrotus purpuratus. Variants csH2A, alpha H2A, beta H2A, gamma H2A, delta HA, H2AF./Z, alpha H2B, beta H2B, and gamma H2B showed up to sevenfold differences in level of monoubiquitination between variants, and indiv...
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HeLa cells were transfected with recombinant DNAs containing the embryonic histone gene repeat of P.miliaris (h22) inserted in either orientation into a pBR-SV40 vector. After 2 to 3 days cytoplasmic RNA was analyzed for authentic sea urchin histone gene transcripts. The correct 5' termini of all five histone genes were detected, three (H2B, H2A and H3) at relatively high levels. In contrast, t...
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DNA fragments comprising units of the repeated histone genes form the sea urchins Psammechinus miliaris and Echinus esculentus were placed under the control of bacteriophage Lambda promoters by cloning into lambda replacement vectors. Although promoter-like regions exist within the cloned fragments, transcription of the histone genes is controlled mainly, but not exclusively, by lambda PL promo...
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Three aspects of early sea urchin development are reviewed, and conclusions derived that lead to a unified concept of how the initial specifications of differential gene activity may occur in this embryo. i. The embryo has an invariant cell lineage, and the lineage founder cells can be considered as regulatory spatial domains. That is, from each of these cells descend clones of progeny the memb...
متن کاملPolycomb group gene expression in the sea urchin.
Embryonic development in metazoan animals involves a remarkable degree of both genetic and epigenetic regulation. Polycomb group (PcG) genes are essential for initiation and maintenance of epigenetic developmental regulation. This report identifies homologous PcG genes representing the core components of three known Polycomb repressive complexes in the sea urchin Strongylocentrotus purpuratus. ...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1981
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.1.7.661